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Batman investigates Scarecrow's presence, and searches for him in a rooftop safehouse, but instead finds and saves Poison Ivy, who was being held captive by Scarecrow's henchmen.
In a makeshift Batcave in Gotham's Clock Tower, Barbara Gordon is collecting information on Scarecrow's new toxin, and traces its production to Ace Chemicals.
On Halloween night, Scarecrow unleashes his newest strain of Fear Gas in a diner, and threatens to release it throughout Gotham.
Six million civilians are evacuated, but the city's criminals and supervillains stay behind, leaving Commissioner James Gordon and the Gotham City Police Department outnumbered.
Batman and Gordon proceed to meet Scarecrow on the building's roof.
Gordon apologizes for his anger and says he has never forgotten how he met Bruce Wayne on the night of his parents' death, since he now knows Batman's identity.
Escaping from Ace Chemicals, Batman discovers that Barbara has been abducted by the Arkham Knight.
Nine months after the Joker's death, crime rates in Gotham City have dramatically declined, but Batman anticipates a new threat and maintains a vigil over the city.
Before his death, the Joker sent his mutated blood to Gotham's hospitals, and several patients were infected.
Batman and Robin free the hostages and capture Harley, but the hostages are killed by Henry Adams, a mutated patient who has fully succumbed to the mutations and desires only the best of the infected to be the new Joker.
When he confronts Batman, Henry inexplicably shoots himself, and Robin realizes that Batman himself is also infected with the Joker's blood.